Biologiical Activities of Cream Containing Curcuma mangga Extract

Abstract

Curcuma mangga Valeton & Van Zijp (Zingiberaceae family) is an annual plant. The rhizomes of this plant are traditionally used for the treatment skin diseases, womb healing and elixir. Pure compounds from C. mangga are diterpenes and curcuminoids which have anti-inflammatory property. In this study, the cream formulation was prepared by using the most stable cream base (formula 2) selected from the 3 preparations of cream base. The most stable cream base was chosen to mix with 2-10% w/w of C. mangga extract. After that, the physical and chemical properties of formulation were investigated as well as evaluated biological activities (anti-oxidant, anti-inflammatory and wound healing effects). The results showed that cream containing C. mangga showed good consistency, homogeneity, pH range of acid value (5-6) and stable after heating-cooling test. For antioxidant activity, cream containing C. mangga exhibited mild inhibition of DPPH radical scavenging (IC50 value >100.0 μg/ml) and cream containing C. mangga protected intracellular free radical from measuring with H2O2-induced oxidative stress. The effect of cream containing C. mangga were examined on LPS-responses in murine macrophages RAW 264.7 cell line. Cream containing 10% w/w C. mangga before and after heating-cooling test (IC50 and 37.9 μg/ml) inhibited inflammation better than cream containing 5% (IC50 = 42.9 and 44.7 μg/ml) and 2% C. mangga (IC50 = 49.1 and 49.6 μg/ml), respectively. In addition, the anti-inflammatory effect of this formulation was better than the positive control (diclofenac gel) with IC50 value of 54.3 μg/ml. The result of wound healing found that cream containing C. mangga before and after heating-cooling test at 1 and 3 μg/ml increased HDF cells proliferation by MTT assay (102.2-110.0%) more than other concentrations (10, 30 μg/ml) while cream containing 5% w/w C. mangga extract before and after heating-cooling test at 3 μg/ml enhanced the cells movement at 36 h (% cell migration = 80.1 and 75.2) followed by 2% (% cell migration = 78.7 and 73.5) and 10% w/w (% cell migration = 76.4 and 72.8). Consequently, cream containing C. mangga extract could reduce inflammation and increased cell viability and recovered the wound. = 34.1