การพัฒนาอุปกรณ์ตรวจวิเคราะห์แบบกระดาษร่วมกับอนุภาคนาโนทองเซนเซอร์แบบตรวจวัดสีสำหรับวิเคราะห์สารไซบูทรามีนในตัวอย่างผลิตภัณฑ์เสริมอาหารลดน้ำหนัก

Abstract

This work reports on the development of paper-based analytical device based on gold nanoparticle sensor for colorimetric detection of sibutramine in adulterated herbal slimming supplements. The determination of sibutramine is based on the aggregation of citrate-stabilized gold nanoparticles sensor induced by sibutramine present in the sample, resulting in the color of gold nanoparticles change from red to blue. The color change on a filter paper can be measured both by the naked eyes and a digital camera in 1 min. The morphology of the citrate- stabilized gold nanoparticles on paper-based analytical device was investigated using scanning electron microscopy (SEM) with energy dispersive X-ray analysis (EDX). Method development was investigated including sample and gold nanoparticle sensor ratio and interaction time. Under the optimized experiment conditions, a linear calibration curve for the sibutramine in the range of 30 to 100 μM (R2 = 0.9864) was obtained. Limit of detection (LOD) and limit quantification (LOQ) were 10 and 30 μM, respectively. The intra-day and inter-day precisions were 0.42-1.21% RSD and 1.27-2.27% RSD, respectively. The sample recovery of sibutramine analysis ranged from 94.67 to 112.41%. A paper-based analytical device can be stored for at least 3 months in a freezer (4 °C and -18 °C). The proposed method was applied for the determination of sibutramine in adulterated herbal slimming supplements samples. The developed method agreed well with the spectrophotometry method using the paired t-test (P > 0.05, n=3). This method is portable, cost-effective, sensitive and low-sample and reagent consumption. It can be used as an alternative method for the analysis of sibutramine which will be useful in the forensic investigation.