การใช้ไรแดงตรวจคัดกรองปริมาณสาร ได-(2-เอทธิลเฮ็กซิล) ทาเลตที่ถุกชะออกจากถุงบรรจุเลือดและขวดบรรจุน้ำดิ่มชนิดใส

Abstract

The objective of this study was to develop a screening test for concentration of DEHP leached from blood containing bags and PET bottles by using an acute toxicity of the leachate on Moina macrocopa as a replacement for a standard chemical analysis method. This study consisted of 1) Toxicity tests of leachate from blood containing bags and PET bottles on Moina macrocopa. 2) Determination of DEHP leached from blood containing bags and PET bottles. 3)Optimization of the condition for a screening test of concentration of DEHP leached from blood containing bags and PET bottles. It was found that the death percentage of Moina macrocopa in leachate from blood containing bags and PET bottles varied with the dilutions of the leachate. Chemical analyses revealed that the average DEHP concentration leached from blood containing bags were between 14.80 35.13 mg/L of which 3 out of 4 brands had DEHP exceeded the Ministry of Industry's standard limit (15 mg/100 ml of solvent). The average DEHP concentration leached from PET bottles were between 0.0048 - 0.0141 mg/L of which 6 out of 8 samples had DEHP exceeded the USEPA's drinking water standard limit (0.006 mg/L). It was found that the average % death of Moina macrocopa at t=24 hr from 10 replications of toxicity test of leachate with 1-fold dilution gave the highest sensitivity and specificity. In conclusion, Moina macrocopa could be used for screening of DEHP concentrations exceeding the standard limits of leachate from blood containing bags and PET bottles.