การแสดงออกของเส้นใยประสาท และ PAR2 ต่อการตอบสนองของการอักเสบเนื้อเยื่อในฟัน

Abstract

OBJECTIVES: In this study, we demonstrate of neuronal profiles and PAR2 expression in deciduous dental pulp at the various stages of dental carious lesions. MATERIALS AND METHODS: Human deciduous dental pulps were used: three from carious lesion-free teeth (group 1), three from limited stage teeth (group 2), two from advanced stage teeth (group 3), and two from pulpal exposure stage of deciduous teeth (group 4). Pulp sections were submitted to immunostaining procedure with fluorescence labelling, the tissues being fixed and incubated with well- characterized monoclonal and polyclonal antibodies against B-tubulin III and PAR2 epitopes. Comparisons among the groups of the quantitative scores for each neuronal PAR2 were analyzed using one-way ANOVA and individual groups were compared by Tukey post hoc test (p < 0.05). This study was approved by the Ethics Committee at the Faculty of Dentistry, Prince of Songkla University (EC6004-10-P-LR) and registered in the Thai Clinical Trials Registry (TCTR20190308001). RESULTS: The immunofluorescence quantification exhibited significantly increase of neuronal fibers in order to respond to the carious penetration. The neuronal fibers show decreasingly to the progression of the dental caries, in the coronal 1/3 region. Moreover, PAR2 also decrease to the progression of lesions. CONCLUSION: PAR2 could play an important role in mediating pulpal inflammation. Moreover, PAR2 were found dramatically decrease associated to the degree of carious penetration. In this study, the neuronal PAR2 also found decreasingly, especially in the carious exposed pulps. This could suggest the neuronal system that exert of bacterial infections.