ยาหกสิ่ง: สารสำคัญ ความเป็นพิษ และรายงานผู้ป่วยที่ได้รับการรักษาด้วยยาหกสิ่ง

Abstract

The aims of this thesis were to investigate a chemical fingerprinting of Ya-Hok-Sing using LC–QTOF–MS method, to assess the toxicity of Ya-Hok-Sing in monkey kidney cells (vero cells) and mouse fibroblast cells (L929) using MTT assay and to follow up on the treatment results as well as assess the quality of life before and after treatment of Ya-Hok-Sing in five patients using the WHOQOL BREF- THAI assessment tool. The MS‑TIC of Ya-Hok-Sing showed the presence of 36 compounds, including 21 phenolics and 15 organic acids. In vitro toxicity test demonstrated that Ya-Hok-Sing had non-toxic effect on both types of the normal cells at the highest concentration tested of 80 µg/ml. The case series result showed that all 5 patients had similar symptoms: high fever, sore throat, cough, muscle pain, headache, fatigue, and body temperature at 38.42± 0.36 °C. Three out of five patients tested positive for COVID-19. Two out of five patients were diagnosed for influenza by a folk healer. During the first 2 days, each patient takes 90 ml of Ya-Hok-Sing every 3 hours. Day 3 onwards, each patient takes Ya-Hok-Sing 3 times (90 ml each) daily before meals for 1 week. If the patients still have symptoms, the drug can be last on day 14. The results showed that all patients had better clinical outcomes within 3-5 days and recovered from fever and co-morbidities within 7-10 days. Quality of life assessment showed that the patients had better quality of life scores after treatment (106.40 ± 7.90), compared to before treatment (62.00 ± 5.34).