Green Synthesis of Silver and Zinc Oxide Nanoparticles using Mimusops elengi Linn. Leaf Extract and Cytotoxicity

Abstract

Green synthesis is one of method for synthesize nanoparticles using biological material. Metal nanoparticles in this research will be produced by synthesis of M. elengi leaf extract that combine with metal precursor to make silver nanoparticle (AgNP) and zinc oxide nanoparticle (ZnONP). Characterization of metal nanoparticle used FTIR, UV-Visible spectrophotometer, XRD and TEM. Application of metal nanoparticle were antioxidant and cytotoxicity against colon cancer, Caco-2. Silver nanoparticles (AgNP) revealed that it has mostly round shapes with the particle size of AgNP was 16.84 nm - 33.67 nm or the average of particle size was 22.12 nm. Zinc Oxide Nanoparticles (ZnONP) characterization revealed that it has mostly round shapes with the particle size was 18.56 - 40.04 nm or the particle size was 28.44 nm in average. Application as antioxidant based on ECso value for extract, AgNP and ZnONP by DPPH assay get the result 139.97 mg/L, 237.33 mg/L and 5380.20 mg/L. The other method by ABTS assay have the result 37.34 mg/L, 215.45 mg/L and 2785.41 mg/L. The last method for FRAP assay showed the value 4.03 μM FE/ mM extract, 0.97 μM FE/ mg extract and 0.09 μM FE/ mg extract. Cytotoxicity of extract and nanoparticles (AgNP and ZnONP) showed that only extract at 2000 ppm has potential against colon cancer (Caco-2 cell) but no effect of nanoparticles (AgNP and ZnONP).