ผลของสารประกอบซิลเวอร์ชนิดต่าง ๆ ต่อจุลชีพในช่องปาก ความเป็นพิษต่อเซลล์เยื่้อบุช่องปากมนุษย์และการหยุดยั้งฟันผุในแบบจำลองฟัน

Abstract

Dental caries is the most common chronic infectious diseases. Scientist attempted to study some substances that will be used to inhibit the progression of dental caries. Silver diamine fluoride which is commonly used in many countries. However, the major disadvantage of Silver diamine fluoride is black stained on tooth surface. This study aimed to evaluate the antibacterial impact of the three silver compounds; 1.) Silver nitrate (AgNO3) 6350 ppm 2.) Silver oxide (Ag2O + NHF) 12700 ppm and 3.) Silver complex (Ag complex) 100 ppm, on five oral pathogenic bacterial strains: Streptococcus mutans, Streptococcus sobrinus, Actinomycosis actinomycetemcomitans, Candida albicans, and Enterococcus faecalis in planktonic and biofilm conditions. The three silver compounds were tested by various methods included: Agar diffusion method, Minimum inhibitory concentrations (MIC), Minimum Bactericidal Concentration (MBC), Biofilm inhibition, and Biofilm eradication. The result revealed Ag2O + NHF and AgNO, had an antimicrobial effect to all of tested bacterial strain. While E. faecalis and Actinomycosis actinomycetemcomitans was not affected by Ag complex in planktonic and biofilm conditions. For the cytotoxicity test on humans oral mucosa (Keratinocyte cell line: H357), the results showed Ag2O + NHF and AgNO, were 100% cells cytotoxicity. Whereas Ag complex at 0.0475 ppm were 47.08% cells cytotoxicity. Effect of the silver compounds in inhibition of human tooth demineralization investigated by MicroCT scanning. The evaluation of linear attenuation coefficients (LAC) from 40 molar teeth showed the percentage of demineralized inhibition were 25%, 45% and 15% of AgNO,, Ag2O + NHF, and Ag complex groups, respectively. Moreover, tooth discoloration after applied the silver compounds at 1, 7 and 14 days showed the maximum rate of tooth discoloration occurred at 7 days. Ag2O + NHF had the highest discoloration followed by AgNO, and Ag complex. This study indicated that Ag2O+ NHF and AgNO, might be potential caries arresting agents for the oral cavity given their desirable properties.