การผลิตชีววัสดุพอลิเมอร์ด้วยกระบวนการไมโครบับเบิลสำหรับวิศวกรรมเนื้อเยื่อกระดูก

Abstract

from OPS and OPT through SSF process was 0.12 Baht/g-LA. The repeated SSF was performed for five cycles for efficient utilization of OPT. The LA production by L. acidophilus TISTR 1338 using this strategy remained high at 63.05±0.44, 71.41±1.07, 67.21±1.71, 56.21±1.67 and 50.48+0.47 g/L, respectively. The recovery of lactic acid using strong base anion resin of Amberlite IRA-402 was performed. The adsorption isotherms were closely predicted by the Freundlich model. The fermentation broth containing lactate was loaded onto the column adsorption at the flow rate of 2.0 mL/min and desorbed by 1 M HCl at the flow rate of 2.0 mL/min resulted in percent recovery of 24.1% from fermentation broth. In addition, this study has explored the possibility to use OPT-PA as a carrier for immobilization of cellulase enzyme by physical adsorption resulting in immobilization efficiency of 79.06%. Comparative evaluation of the free and immobilized enzyme showed that the immobilized enzyme was more thermostable than the free form. This study has provided a promising strategy for efficient use of cellulosic agricultural wastes for production of organic acids and immobilization of enzyme.