การย่อยสลายร่วมไร้อากาศระหว่างน้ำทิ้งจากการหมัก เอบีอี และของเสียจากการผลิตไบโอดีเซลเพื่อผลิตไบโอไฮเทน

Abstract

A substrate having improper C/N ratio could cause serious inhibition during anaerobic digestion process by high volatile fatty acids (VFA) and total nitrogen- ammonia (TAN) accumulation, which are generated by using a substrate having high and low C/N ratio, respectively. In this investigation, glycerol waste (GW) generated by trans-esterification process for biodiesel production as high carbon content was co-digested with nitrogen rich substrate, acetone, butanol, ethanol fermentation (ABE) wastewater or distillery spent wash (DSW) for hydrogen and methane production via two-stage anaerobic process under thermophilic conditions (55 °C). Subsequently Bio-hydrogen and methane potential (BHP and BMP) assays adding with various GW and ABE wastewater at mixing ratios of 0:10, 1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, 9:1, and 10:0 (VS basis) at initial organic concentration of 10 g-VS/L were evaluated. The highest BHP of 167.0 mL-H2/g-VS was achieved from a batch digestion having glycerol waste to ABE wastewater mixing ratio of 5:5, whereas insignificantly different BMPs in a range of 449.0-513.0 mL-CH/g-VS were obtained from all mixing ratios. DSW and GW at mixing ratio 5:5 (VS basis) was continuously fed to the continuous stirred tank reactor (CSTR) for hydrogen production and subsequently to the up-flow anaerobic blanket (UASB) for methane production under thermophilic conditions (55 °C). Experimental results showed that the hydrogen production of 82.3 mL-H2/g-VSadded was achieved at a 4-day HRT. Effluent from hydrogen stage was then fed to produce methane in the UASB reactor at consecutive 21- and 18- day! 426 mL-CH (ADM-1) Padded modified with methane yields of 356 mL-CH/g-VSadded and sees The original anaerobic digestion model no. 1 ance describing co-digestion of glycerol waste and vinasse in the co-digestion by including corresponding biochemical and physic- The developed model could be properly used to predict the the two-stage anaerobic process for co-digestion of GW and DSW. hydrogen and methane yields obtained demonstrate that co-of ABE wastewater/DSW could potentially provide effective degradation of matters and generation of gaseous bio-fuel in a form of mixed hydrogen and methane, so-called biohythane.