การหาคุณลักษณะทางกายภาพของสารเพิ่มน้ำเลือดที่มีแป้งมันสำปะหลังดัดแปรด้วยกรดร่วมกับวิธีไฮดรอกซีโพรพิเลชัน

Abstract

Plasma expanders (PEs) are administered fluids to restore blood volume when massive blood loss occurs. Modified tapioca starch might be an interesting source for PE such as hydroxypropyl, acid-thinned tapioca starch. The objectives of this study were to prepare and characterize plasma expanders from hydroxypropyl, acid-thinned tapioca starch and to compare the physical properties of hydroxypropyl, acid-thinned tapioca starch-based PEs with Voluven (6% hydroxyethyl starch 130/0.4; 6% HES 130/0.4). Hydroxypropyl, acid-thinned tapioca starch was prepared by the etherification with propylene oxide to acid modified tapioca starch. Hydroxypropyl, acid-thinned tapioca starch was mixed with 0.9% sodium chloride solution to obtain 8 concentrations (1, 3, 4, 5, 6, 7, 8 and 10% weight per volume) of PE. Physical properties were determined such as turbidity, pH, colloid osmotic pressure (COP) and viscosity. The effects of PE on morphology of red blood cells and blood plasma properties were investigated. The results showed that the physical properties of hydroxypropyl, acid-thinned tapioca starch-based PE depended on the concentration of hydroxypropyl, acid-thinned tapioca starch. Furthermore, hydroxypropyl, acid-thinned tapioca starch-based PE had higher turbidity and pH than 6% HES 130/0.4. A change of shape and surface of red blood cells were observed when mixing blood with hydroxypropyl, acid-thinned tapioca starch-based PE. The COP of 8% hydroxypropyl, acid-thinned tapioca starch-based PE was closed to 6% HES 130/0.4. In addition, the viscosity of 6% hydroxypropyl, acid-thinned tapioca starch-based PE was also closed to 6% HES 130/0.4. However some properties of 6% hydroxypropyl, acid-thinned tapioca starch-based PE were different compared to 6% HES 130/0.4. Therefore, it is necessary to investigate on further modification of hydroxypropyl, acid-thinned tapioca starch to obtain proper physical properties of PE.