ผลของโพรดิจิโอซินจากแบคทีเรีย Zooshikella ganghwensis ที่แยกได้จากฟองน้ำทะเลในกุ้งขาว (Litopenaeus vannamei)

Abstract

In this study, a total of 8 pigmented bacteria (PSU-KSAAHRC MS1-8) were isolated from five species of marine sponges. Of these 8 isolates, one bacterial isolate PSU-KSAAHRC MS2 from marine sponge Haliclona sp. from Satun provinces, produced highest pigment of 3,140.23± 464.80 μg/g. The red pigment found in the bacterial isolate PSU-KSAAHRC MS2 exhibited maximum absorption ranged from 535-539 nm and mass spectrometry revealed molecular weights of 322.19-324.20 Da, which were identified as prodigiosins. Based on morphological and biochemical characteristics as well as phylogenetic analysis, this bacterium was identified as Zooshikella ganghwensis. Optimization of prodigiosin production at various conditions showed that Z. ganghwensis PSU-KSAAHRC MS2 produced maximum prodigiosin at temperature of 30 °C and pH 8. Investigation of dietary administration of Z. ganghwensis PSU- KSAAHRC MS2 at 0, 1, 3 and 5 g/kg diet in Pacific white shrimp (Litopenaeus vannamei) indicated that dietary supplemented Z. ganghwensis PSU-KSAAHRC MS2 had no effect on growth performance of Pacific white shrimp infected with Vibrio parahaemolyticus. However the highest survival rate (61.67±24.66 %) was observed in shrimp fed with dietary supplemented Z. ganghwensis PSU-KSAAHRC MS2 at 1 g/kg diet. Moreover, the highest total hemocyte count (3.73±0.70x10' cell/ml) phenoloxidase activity (19.57±1.23 unit/min/mg protein) phagocytic activity (54.33±0.76 %) phagocytic index (42.82±0.66) and catalase enzyme activity (73.40±24.6 unit/mg protein) were found in shrimp fed with dietary supplemented Z. ganghwensis PSU- KSAAHRC MS2 at 1 g/kg diet which was significantly different from other treatments (p<0.05). Histopathological findings revealed normal hepatopancreatic tissue observed in shrimp fed with dietary supplemented Z. ganghwensis PSU-KSAAHRC MS2 at 1 g/kg diet when compared to other treatments, indicating that prodigiosin from Z. ganghwensis treat the shrimp from V. parahaemolyticus infection.