ความแปรปรวนทางพันธุกรรมของแพะเนื้อลูกผสม "ทรัพย์-ม.อ. 1" โดยใช้ไมโครแซทเทลไลท์ และไมโทคอนเดรีย ดีเอ็นเอ

Abstract

The objective of this study was to investigate the genetic variation of crossbred meat goat Sup-PSU 1 (50% Thai native and 50% Anglo-Nubian goats) that developed by Ruminant Research and Development Center, Faculty of Natural Resources, Prince of Songkla University using microsatellites and mitochondrial DNA (D-loop). Forty eight Sup-PSU 1 goats together with thirty Thai native goats and thirty Anglo-Nubian goats were used in this study. Results from the 11 microsatellites showed observed and expected heterozygosity value of Sup-PSU 1, Thai native and Anglo-Nubian goats were 0.296 (0.679), 0.338 (0.682) and 0.329 (0.621), respectively. All groups of goat populations showed high polymorphism. The comparision of subpopulations with the total populations (F,T), a low value of FST (0.138) was indicated. This was probably related to the small subpopulation from three groups of goat. Also, this study revealed the polymorphism of three groups of goats. PCR-RFLP of mitochondrial DNA showed 2 patterns of genotype, GG and GC. The CC genotype was not indicated in all groups of goat, while GC was not found in Thai native goat. This may be due a specific allele C in Anglo-Nubian goats. Moreover, Sup-PSU 1 and Anglo- Nubian goats were in equilibrium of Hardy and Weinberg. Nevertheless, native goat could not be tested by Hardy and Weinberg equilibrium. Due to the only one allele and genotype pattern were found in the native group. When using the genetic distance from the microsatellite and mitochondria DNA to construct a phylogenetic tree using Neighbor Joining from program NTSYS V. 2.1. Two groups of goats could be divided. First group consisted of Sup-PSU 1 and Anglo-Nubian goats, while the second group was a native goat. From the results could be concluded that Sup-PSU 1 had high polymorphism. In addition, Sup-PSU 1 goat had a closer relationship with Anglo-Nubian goat than Southern Thai native goat.