Biological Activity of Chemical Constituents from Cinnamomum porrecyum (Roxb.) Kosterm

Abstract

Cinnamomum porrectum (Roxb.) Kosterm (Theptaro) is a aromatic tree. Mostly of the species are in the throughout southern Thailand and have medicinal properties such as helping to resolve diarrhea, flatulence, dizziness and vomiting. The purpose of this study was to isolate pure compounds from crude extracts the roots and barks of C. porrectum and assess for antibacterial activities, plant pathogenic fungal and antioxidant activity of crude extracts and pure compounds from the roots and barks. Air-dried roots and bark of C. porrectum to maceratied with dichloromethane and acetone. The results revealed that the pure compounds could be isolated from crude extracts the roots and barks and contains phenylpropanoid derivatives.Six compounds wereisolated including Safrole(CP1), 3,4 Methylenedioxy cinnamaldehyde (CP2), n-Butyl ferulate (CP3), 3-(3,4 Methylenedioxyphenyl)-1,2-propanediol ( CP4) , Elemicin ( CP5) and 3,4-(Methylenedioxy) cinnamyl Alcohol ( CP6) . Their structures were characterized of these compounds based on 1 H and 13C NMR spectroscopy data and compared with data of the previously reported. The crude extracts of dichloromethane, acetone and all six compounds have been evaluated for antibacterial activity of 4 bacteria species: Staphylococcus aureus (ATCC25923), Bacillus cereus (ATCC11778), Pseudomonas aeruginosa (ATCC27853) and Escherichia coli (ATCC25922). The results revealed that dichloromethane extracts of the roots and barks were showing a good inhibition in the region of B. cereus (ATCC11778) values of 22.33 and 17.00 mm respectively. CP6 showed activity in inhibitingS. aureusand B. cereus with MIC values of 1,000 and 2,000 µg/ml respectively. more effectively than CP4 which showed inhibition potential against S. aureus and B. cereus with equal MIC values of 2000 µg/ml for antifungal activity. To investigate the plant pathogenic fungal, The results were found that dichloromethane root extracts from C. porrectum at concentrations of 10, 5, 1 mg/ml showed best activities to inhibit the growth of A. alternata at 48.86 – 100.00 percent. All compounds exhibited weak activity against A. alternata with MIC values of greater than 100 µg/ml. Antioxidant activity assays by using DPPH (1, 1-diphenyl-2- picrylhydrazyl) radical scavenging activity assay method, test results found that n-Butyl ferulate has the best antioxidant activity. The inhibitory concentration at 50%, IC50 values of CP3 were 55.52 mg/ml.