Effect of Bone Marrow Concentrate on New Bone Formation in Rabbit Calvarial Defect

Abstract

Objective: The aim of the present study was to compare new bone regeneration in rabbit calvarial defects treated by autogenous bone, beta-tricalcium phosphate alone (B-TCP), in combination of beta-tricalcium phosphate and bone marrow aspirate concentrate (B- TCP+BMAC), and empty defect. Materials and methods: Two critical size defects were created on the calvarium of 15 male white New Zealand rabbits. The clinical evaluation and Micro-computed tomography (micro-CT) was used for evaluation of new bone regeneration at 2, 4 and 8 weeks after surgery respectively. Results: The experimental models showed good clinical biocompatibility to all groups of the materials. The highest bone formation was achieved with autogenous bone treatment, and it was higher significantly than other groups. Bone formation in B-TCP and B- TCP+BMAC groups were not significantly different, but higher than empty defect. The quantity of residual graft materials were reduced gradually and new bone formation were increase with time in both groups. Conclusion: This study showed that beta-tricalcium phosphate added with bone marrow aspirate concentrate could be one of the alternative material of choice for new bone formation in rabbit calvarial defect.