การประเมินผลของสารสกัดจากใบกระท่อมต่อการถอนมอร์ฟีนในระยะยาวในหนูทดลอง

Abstract

Chronic discontinuation of opioids usage leads to emotional changes. Especially of negative emotional states, which is a psychiatric symptom detected during drug withdrawal. In molecular studies showed that drug abuse cause disturbance of function of molecules involved in memory pathway in the brain. The purpose of this study was to evaluation of extract from kratom leaves (Mitragyna speciosa (Korth.) Havil) in long lasting morphine withdrawal in mice. Mice were divided into control group (n=8), morphine-withdrawal groups (n=8) and kratom group (n=8). Mice received an escalating dose of morphine (10, 20, 40, 60, 80, 100 mg/kg, twice daily at 09.00 and 16.00) or saline for 8 consecutive days. In the during withdrawal period, a saline is injected instead of a morphine injection. The extract from kratom was administered during withdrawal period 30 minutes before the behavioral test at 80 mg/kg for 5 days. The results showed that repeated treatment of morphine dramatically decreased the body weight of morphine-treated mice, assessed on day 8, compared with saline-treated (control) group. In the during withdrawal period, mice were more exhibited anxiety related behaviors and depressive-like behaviors were increased. When treated with extract from kratom leaves at 80 mg/kg, it reduced both anxiety-related behavior, depression-like behavior.