บทบาทของไพรไบโอติก Lactobaacillus paracasei SD1 Lactobacillus rhamnosus SD11 และ Lactobacillus gasseri SD12 ต่อความสัมพันธ์โฮสต์และจุลชีพในลำไส้ของแมลงหวี่

Abstract

Our previous studies showed that Lactobacillus paracasei SD1, Lactobacillus rhamnosus SD11 and Lactobacillus gasseri SD12 have been considered as the potentially oral probiotics for improvement oral health. However, the effects of these oral probiotics on general health have not been studied. The aim of this study was to evaluate the effect of L. paracasei SD1, L. rhamnosus SD11, L. gasseri SD12 and L. rhamnosus GG, the commercial probiotics, on growth and on the establishment of probiotics in the fly gut. For evaluate the effects of probiotics on the growth, the fly's eggs were introduced onto the food with probiotics in test groups and without probiotics in control group. They were fed with these food until adults. The morphogenesis and development rate of flies were evaluated and the weight of 7-day-old adult flies were measured. The study of the effect of probiotics on weight and the establishment of probiotics in the gut of adult fly, adult flies were reared in food with probiotics in test groups and without probiotics in control group. Flies' weight and the probiotics in their gut were evaluated on day 1, 2, 3 and 5. The study revealed that the duration of morphogenesis of probiotics fed groups were faster, and the development rate of flies from larva to pupa and adult were also higher in probiotics groups than control group. However, the development rate in L. gasseri SD12 group was similar to control group. The mean body weight of the fly reared with probiotics since larva stage was statistically significant higher than control group. However, the effect of probiotics on body weight in adult fly was not different from control group. The number of probiotics bacteria were increased in all probiotic groups and reach to the highest point in the third day, except L. gasseri SD12 group, which had lower level of gut probiotics than other probiotics groups throughout the study. These finding can be concluded that feeding with selected probiotics can promote effect on growth in a strain-dependent manner.