การประยุกต์ใช้ Trichoderma harzianum ChM1.1 เพื่อควบคุมโรครากขาวของยางพารา

Abstract

White root rot of rubber is one of the most important diseases affecting this crop worldwide. The objective of this study was to evaluate the capacity of Trichoderma harzianum ChM1.1-enriched biofertilizer to minimize white root disease of rubber tree. Trichoderma harzianum ChM1.1 was screened for their antifungal activity against 60 isolates of Rigidoporus microporus by dual culture assay. The results showed that T. harzianum ChM1.1 was able to inhibit the mycelial growth of R. microporus with percentage inhibition between 74.95 - 93.93 %. T. harzianum ChM1.1-enriched biofertilizers were tested for their ability to inhibit the mycelial growth by suppressive soil technique. The results demonstrated that T. harzianum ChM1.1-enriched biofertilizers B and G showed no significant inhibition of the mycelial growth of R. microporus PM-5 2 with 78.42 and 78.33 % inhibition, respectively, as compared to the treatment with carboxin. In greenhouse experiments, application of T. harzianum ChM1.1-enriched biofertilizer B significantly reduced the incidence of white root rot fungal disease compared with untreated controls. Therefore, the use of T. harzianum ChM1.1-enriched biofertilizer may be an alternative or complementary strategy for the control of this disease.