An in vitro evaluation of the expression of IL-1B and TNF-A in human periodontal ligament fibroblasts after exposure to mechanical vibration combined with combined with compressive stress

Abstract

The use of vibration to accelerate the tooth movement is very interesting. However, the studies on the effect of vibration on accelerated tooth movement still remain controversial. Moreover, the mechanisms of mechanical vibration to stimulate the tooth movement are not known. Objectives: To investigate the effects of mechanical vibration combined with the compressive force in human PDL cells via the gene and protein expression of IL-1ẞ and TNF-α. Materials and Methods: Human PDL cells derived from first premolars were randomized into six groups: CT groups (unloaded), COM groups (compressive stress 2 g/cm3), Vi groups (vibration: 30 Hz and 60 Hz), and CV groups (compression & vibration). Real-time PCR was used to investigate the gene expression of IL-1ẞ and TNF-α and ELISA was used to quantitate the protein levels of IL-1ẞ and TNF-α. Results: The expression of IL-1ẞ and TNF-α in COM and CV groups (30 & 60 Hz) increased significantly when compared with CT groups and highest expression in CV group of 30 Hz. While the expression of IL-1ẞ and TNF-α decreased in all Vi groups and was less than the control group. Furthermore, the protein levels of IL-1ẞ and TNF-α in COM groups and all CV groups were higher than CT group but not significant. Conclusions: The mechanical vibration combined with compressive stress can alter only the gene expression of IL-1ẞ and TNF-α but does not change the protein expression of IL-1ẞ and TNF-α in human PDL cells.