การประเมินเสถียรภาพทางพันธุกรรมของต้นหม้อข้าวหม้อแกงลิง(Nepenthes mirabilis) ที่ขยายพันธุ์ในหลอดทดลองโดยใช้เทคนิคโมเลกุล และสัณฐานวิทยา

Abstract

Nepenthes mirabilis is dioecious carnivorous plants, which has evolved beautiful pitcher. It has been taken from the forest resulted in a lost of a large number. Therefore, propagation for conservation is neseesary. Plant tissue culture is a proper way for mass propagation. However, the rapid multiplication or long-term cultures may affect for somaclonal variation induction. Thus, this research was aimed to assessment of somaclonal variation among micropropagation of N. mirabilis in three regenerations by using RAPD, ISSR and SSR technique. The result revealed that 3 out of 16 RAPD primers screening showed distinctly different banding DNA patterns in 2 and 3 regenerations. In the case of ISSR, 4 out of 15 primers screening showed different banding DNA patterns in all regenerations. Similarity to SSR marker that 4 out of 5 SSR primers screening, revealed distinctly different banding DNA patterns in all regenerations. The result of molecular marker was confirmed with morphological characteristics that showed their differences among somaclones on leave and stomata characteristics compared with mother plant. The present study demonstrates molecular techniques together with morphological characteristics are an efficient tool for the assessment of somaclonal variation of in vitro plants.