Effects of Leukocyte-Platelet-Rich Plasma on Accelerated Orthodontic Tooth Movement in Rabbits

Abstract

Introduction: A need of developing new methods to accelerate tooth movement has been recently investigated. Leukocyte-platelet-rich plasma (L-PRP) has been introduced as a source of autologous growth factors and secretary cytokines that are involved in bone remodeling and inflammatory process during orthodontic tooth movement. Thus, the administration of L-PRP could be used in order to accelerate tooth movement and reduce treatment time. However, no data are available on the use of L- PRP to accelerate orthodontic tooth movement. Objectives: To determine the effects of a local injection of L-PRP on the rate of orthodontic tooth movement, alveolar bone changes, and osteoclastic activity in rabbits. Materials and methods: Twenty-three male New Zealand white rabbits were included in a split-mouth design. Tooth movement with a 100-gram nickel-titanium closed-coil spring was performed on the maxillary first premolars. L-PRP was injected submucosally at the buccal and lingual areas of the first premolar in one random side of the maxilla and the other side served as the control and received normal saline. The amount of tooth movement was assessed on three-dimensional digital models at day 0 as the baseline and on days 3, 7, 14, 21, and 28. The bone volume fraction and the osteoclast numbers were assessed at 0, 7, 14, and 28 days by microcomputed tomography (micro-CT) and histomorphometric analysis, respectively. Results: The L-PRP group showed a significantly increased cumulative amount of tooth movement at all observed periods. However, a significantly higher rate