ประสิทธิภาพของ gymnemic acid สารสกัดจากผักเชียงดาที่มีต่อการเปลี่ยนแปลงของหลอดเลือดในไตหนูที่เป็นเบาหวาน

Abstract

Diabetic nephropathy is a complication caused by high blood sugar for a long time, resulting in kidney degeneration, affecting kidney function and leading to chronic kidney disease. Various pathologies in diabetic nephropathy are related to the duration of diabetes and level of blood glucose. Increasing of vascular endothelial growth factor (VEGF) and transforming growth factor beta (TGF-β) are the important causes of endothelial cell dysfunction. In this study, induction of diabetes in male Wistar rat by streptozotocin (STZ). Mice were divided into 5 groups as control group (C), control group was treated with gymnemic acid (400 mg/kg BW) (CGM), diabetic group (DM), diabetic group treated with gymnemic acid (400 mg/kg BW) (GM), diabetic group treated with glyburide (GR) every day for 8 weeks. At the end of 8 weeks, the kidneys were processed by H&E staining, immunofluorescence staining for study the expression of VEGF and TGF-β and vascular corrosion cast technique with scanning electron microscopy. Results were found the increasing in blood glucose, the thickening of segmental arterial walls and angiogenesis in diabetic group. The size of arteries in kidney and glomerulus were decreased in diabetic group. The expression of VEGF and TGF-β were increased in diabetic group. Administration with gymnemic acid showed the reduction of blood glucose, the recovery of blood vessels and glomerulus and decreasing of the expression of VEGF and TGF-β compared with diabetic group. These results have provided the evidence of gymnemic acid that have the potential in reducing blood glucose and attenuating endothelial cell dysfunctions