การเปรียบเทียบความสามารถในการต้านเชื้อและการคงฤทธิ์ของสารประกอบซิลเวอร์ในโพลอกซาเมอร์ 407 เจล และในมาโครกอลผสมโพรพิลีนไกลคอลสำหรับใช้ในคลองรากฟัน

Abstract

The failure of root canal treatment caused by many factors for example, tooth with persistent infection, improper quality of root canal filling material, complication occurred between root canal treatment or improper coronal restoration. Moreover, another factor that affects the failure is microorganisms. Most common microorganisms in case of failed endodontic treatment are Enterococcus faecalis (E. faeculis) and Candida albicans (C. albicans). So during a root canal procedure, the tooth need medicament in order to inhibit growth and eliminate the amount of microbes during treatment. Calcium hydroxide Ca(OH), is the most common medicament has been used in endodontics because of wide range of antimicrobial activity but from previous study found that Ca(OH), less effective against E. fucculis and C. albicans. Thus, chlohexidine has started to be used as an intracanal medicament due to board spectrum antimicrobial effect, substantivity to dentin and low toxicity but chlohexidine has limitation in use because it can interact with sodium hypochlorite that is the most commonly used as irrigant in root canal treatment and will result in the formation of para chloroaniline (PCA) sediment. Nowadays silver has been used in both medicine and dentistry because of its broad spectrum of antimicrobial properties and low toxicity. From these properties of silver so we want to develop as a medicament in root canal to reduce the limitations of the use of chlohexidine. To use silver as an intracanal medicament is required the vehicle. Therefore this study consider using macrogol or polyethelene glycol mixed with propylene glycol (MP) and poloxamer 407 (P407) because the desired properties for vehicle of intracanal medicament. The objective of this study is to compare antimicrobial activity and substantivity of silver compounds in P407 and in macrogolmixed with propylene glycol as an intracanal medicament. This study examine antimicrobial activity of E. faecalis and C. albicans in planktonic condition among silver complex, silver oxide (Ag2O) and silver nitrate (AgNO3) in P407 and in macrogol mixed with propylene glycol compared with 2% chlohexidine gel (Consepsis" V) with agar well diffusion method and select silver compound that the most antimicrobial activity effect when mixed with vehicles for the further study in antimicrobial activity and substantivity in tooth model. Result from agar well diffusion method found that AgNO, is the most antimicrobial activity effect in both E. faecalis and C. albicans in planktonic condition follow by Ag2O and Ag-complex respectively. The result antimicrobial activity in tooth model found MP+AgNO, is the most antimicrobial activity effect same as Consepsis V in C. albicans but in E. faeculis the antimicrobial activity effect less than Consepsis" V. The result of antimicrobial substantivityfound that AgNO, in P407 and in macrogol mixed with propylene glycol dose not have substantivity effect in 2 days after study From antimicrobial activity result suggest that AgNO,in MP can be used as an intracanal medicament in root canal.