การเตรียมและสมบัติของโครงสร้างเลี้ยงเซลล์ที่มีความเข้ากันได้ทางชีวภาพเตรียมจากพอลิแลคติคแอสิด/ไคโตซาน/ผงขี้เลื้อย

Abstract

The objective of this study was to prepare and determine properties of biocompatible composite foams consisted of poly(lactic acid) (PLA), chitosan and rubber wood sawdust (Hevea brasiliensis) for utilizing as scaffold in bone tissue engineering. Foam samples were prepared by compression molding. Azodicarbonamide and zinc oxide was used as a chemical blowing agent and an accelerator, respectively. Poly(ethylene glycol) (PEG) was used as a plasticizer. Foam samples were classified into 3 types: (1) PLA/rubber wood sawdust foams, (2) PLA/chitosan/PEG foams, and (3) PLA/chitosan/rubber wood sawdust foams. Surface treatment of rubber wood sawdust was applied. It was found that cellular structure of foams was closed cells. Factors affecting foam morphology and physical properties (average cell size, void fraction (%VF), and density) were particle size, concentration and surface treatment of rubber wood sawdust, concentration of chitosan and PEG, and molding condition. These factors also affected the mechanical properties and thermal degradation of foams. Foams contained smaller average cell size/%VF and higher density showed higher mechanical properties. The thermal degradation temperature determined from TGA techniquedegradation and cytotoxicity sts were applied to Foam 1 and 2. The In-vitro degradation was reported as % weight loss of samples which tested for 2 months. Noticeably, average cell size, %VF, and % weight loss of both foams. % weight loss of all formulae of depended wasI was significantly lower than that of PLA foam. % weight loss of Foam 1 It could be higher, lower or similar to that of PLA foam. by cell proliferation obtained from MG63, osteoblast-liked cells, days. All of Foam 1 and 2 showed non-cytotoxicity confirmed by theproliferation throughout the cell culture.