การปรับปรุงประสิทธิภาพการผลิตไฮโดรเจนจากพืชน้ำและสาหร่ายขนาดเล็ก

Abstract

Aquatic plants and microalgae in Thailand has rapidly expanded, The degradation of aquatic plants consequently deteriorates the water quality. However, aquatic plants and microalgae have advantages in reducing organic matter in water. Using aquatic plants and microalgae as a substrates in biohydrogen production from dark fermentation can be produced as an alternative energy to reduce the use of fossil fuels with CO2 emissions from combustion which is the cause of global warming. The objective of this research was to evaluate effectiveness of aquatic plants and microalgae pretreatment using microwave radiation to enhance biohydrogen production from dark fermentation. Results illustrated pretreatment of duckweed, water hyacinth, hydrilla and microalgae using microwave irradiation energy intensity of 0 - 99,000 kJ/kgTS, El> 33,000 kJ/kgTS decrease solubilization yield of all substates demonstrating that the microwave irradiation can destroy the lignocellulose structure. The study of specific hydrogenic activity was conducted at 35°C and 55°C pH 5.5 to evaluate the potential of inoculum using different thermal pretreatment. Slow heat showed higher hydrogenic activity than fast heat, glucose and starch could be used to represent the effectiveness of hydrogen production at 35°C and 55°C respectively. Biochemical hydrogen potential assay was performed with slow heat at 55°C pH 5.5 using aquatic plants and microalgae to estimate the effect of microwave energy intensity (El) 13,200; 26,400; 52,800 kJ/kgTS. Microalgae gave highest H2 yield was 26.0 mL/gTS at 13,200 kJ/kgTS, In the subsequent experiment, Microalgae via micro oxygenation with oxygen dose 0, 10 and 15 mLO2/mLinoculum at 168 h, Oxygen dose 10 mLO2/mLinoculum exhibit highest H2 yield was 37.1 mL/gTS. This approach can reduce cost, time and energy consumption during inoculum pretreatment. Thus it could be feasible strategy for sustainable environmental and energy management.