ผลของการกระตุ้นกระแสไฟฟ้าร่วมกับแสงจากไดโอดเปล่งแสงสีแดงต่อการหายของบาดแผลที่ผิวหนัง

Abstract

Wound healing is a biological process which replaces or repairs the tissue damage. Many treatments to induce wound healing have been studied both in vitro and in vivo such as pharmacological treatments and non-pharmacological treatments. Electrical stimulation and photo therapy are considered as non-pharmacological treatments. The objectives of this study are to fabricate a prototype of an instrument that combines an electrical stimulation and photo therapy using red light-emitting diode (LED) for wound healing and to investigate the effects of this combination treatment in the animal model. The electrical stimulator can generate 10μA direct current and it can also generate red light with 660 nm in wave length. In this study, there were 66 Wistar rats divided in 4 groups; control group, electrical stimulation group, light emitting diode group and electrical stimulation combined with light emitting diode group. Wound area on dorsal skin was photographed and calculated for wound healing rate using ImageJ. Furthermore, the amount of collagen and fibroblast growth factor was evaluated on day-7, 14 and 21.Results of this study showed that the wound healing rate at day-14 in a treatment group with electrical stimulation combined with red LED was 60% whereas that in other groups was lesser. However, after 16 days, there was no difference in wound healing rate. From staining results, it support that the treatment groups had better healing rate at day-7 and day-14 when compared with the control group because the color intensity relate to the amount of collagen and fibroblast growth factor were higher. However, there was not much different at day-21 among these groups. It can conclude that the combination of electrical stimulation and red LED provided faster wound healing rate during 10 days after wound occurrence compared with a single treatment as a result of an increase of collagen and fibroblast forming.