การประเมินการระคายเคืองและประสิทธิภาพการรักษาแผลของไฮโดรเจลผสมฟาโกไซโตซิสแอคติเวติงโปรตีนและทรอมโบสปอนดินในหนูทดลอง

Abstract

Wound healing is a complex physiological process. If it is interfered, it will result in the wound healed slowly and eventually become chronic wound. Hydrogel is a polymer that has properties to serve the skin moist. Phagocytosis activating protein (PAP) has the ability to stimulate the process of phagocytosis in white blood cells. Thrombospondin (TSP), a protein that contains epidermal growth factor (EGF) like domain, which stimulates the EGF receptor (EGFR) induces wound healing. Therefore, the purpose of this study was to investigate the irritation and the efficacy of wound healing in rats using hydrogel contain protein in different types and dosage include polyvinyl alcohol and poloxamer (PVA-Poloxamer) hydrogel, hydrogel containing low dose and high dose of PAP or TSP (1 μg/ml, 10 μg/ml respectively), hydrogel containing low dose of PAP and TSP (0.5 μg PAP/ml, 1 μg TSP/ml) and high dose (0.5 μg PAP/ml, 10 μg TSP/ml). The results of the irritation study showed that all 7 formulas did not cause skin irritation in experimental animals with a total score of no more than 2 points and no statistical difference (p < .001). The results of the efficacy of wound healing showed that on day 3 and 6", the wounds were treated with hydrogel containing low dose of PAP had less wound area than those were treated with PVA-poloxamer hydrogel (p <0.05), with minimal wound area throughout the study period (3.76% day 21"). Subsequently, the wounds were treated with hydrogel containing low dose of TSP (4.13%). The results of the histopathology showed that the epithelial cells adhesion and the normal epithelial cells thickness of the wounds were treated with hydrogel containing low dose of PAP and the wounds were treated with hydrogel containing low dose of TSP were faster than other wounds. It was also found that the wounds were treated with hydrogel containing low dose of PAP had the highest levels of TGF-B1 on day 10th and 14" (14.9 ± 0.8, 17.7 ± 1.0 respectively). Subsequently, the wounds were treated with hydrogel containing low dose of TSP (13.4 ± 0.7, 15.1 ± 1.1 respectively), and the wounds were treated with PVA-poloxamer hydrogel (12.3 ± 0.2, 14.6 ± 0.8, respectively). On day 21", the wounds were treated with hydrogel containing low dose of PAP had the lowest level of TGF-ẞ1, followed by the wounds were treated with hydrogel containing low dose of TSP and the wounds were treated with PVA- poloxamer hydrogel (7.1 ± 0.9, 7.8 ± 1.0, 9.7 ± 0.9 respectively). Therefore, hydrogel containing low dose of PAP and hydrogel containing low dose of TSP are promising formulas for the development of new wound dressing materials further.