ประสิทธิภาพของ Gymnemic acid จากผักเชียงดาที่มีผลต่อการเปลี่ยนแปลงของหลอดเลือดในสมองหนูที่เป็นเบาหวาน

Abstract

Diabetes mellitus is a disease that caused by abnormality of producing insulin hormone from pancreas lead to high blood glucose level which known as hyperglycemia. Hyperglycemia can lead to many complication diseases. Stroke is one of the most important complication disease which caused by chronic hyperglycemia. The purpose of this study was to investigate the effects of gymnemic acid from Gymnema sylvestre for restoration and improvement of the main blood vessels of anterior circulation of brain which includes internal carotid artery (ICA), middle cerebral artery (MCA) and anterior cerebral artery (ACA). In this study we used 8 week-old male Wistar rats and divided into 5 groups including control group (C), control treated with gymnemic acid (CGM), diabetic group (DM), diabetic treated with gymnemic acid (GM) and diabetic treated with glybenclamide (GR). After 8 weeks of the experiment, the diabetic treated with gymnemic acid group (GM) showed the restoration of structural changes of blood vessel and micro vascular architecture which decreasing of the thickness of blood vessel wall, increasing the diameter of blood vessel. In western blot analysis indicated that the GM group had a significantly decreased of Vascular endothelial growth factor (VEGF) and Angiopoietin I (Ang-1) expression which associated with the expression of blood vessel damaged indicator and angiogenesis. Therefore, our results suggest that gymnemic acid can improve and restore the blood vessel which damaged by hyperglycemia in Diabetes mellitus.