PSU Knowledge Bank Collection:http://kb.psu.ac.th:80/psukb/handle/2553/12462024-02-29T02:53:14Z2024-02-29T02:53:14ZFormulation optimization and characterization of colistin using sodium deoxycholate sulfate for intravenous administrationBintang, Muhammad Ali Khumaini Mudharhttp://kb.psu.ac.th:80/psukb/handle/2016/192592023-12-20T09:10:33Z2023-01-01T00:00:00ZTitle: Formulation optimization and characterization of colistin using sodium deoxycholate sulfate for intravenous administration
Authors: Bintang, Muhammad Ali Khumaini Mudhar
Abstract: Colistin is still used in multidrug-resistant gram-negative bacteria (MDR-GNB) therapy but the nephrotoxicity and neurotoxicity still become a major setback for its clinical use. Sodium deoxycholate sulfate (SDCS) carrier is used in micelle formulation with colistin to mitigate the known toxicity of colistin. The surface property of colistin and SDCS were evaluated before formulation with the lyophilisation technique. Several physicochemical parameters were evaluated like particle size, zeta potential, morphology, encapsulation efficiency (EE) and release profile. The chemical interactions were analysed using FTIR, NMR and molecular docking. The cytotoxicity of formulations was tested with different kidney cell lines. The in vivo toxicity was carried out in male mice C57BL/6 with colistin and colistin formulation for 7 consecutive days. The physiological changes were observed and measured after treatment. The serum biomarkers were measured including blood urea nitrogen (BUN), creatinine (Cr), superoxide dismutase (SOD), and catalase (CAT). Histopathological alterations in mice organs were analysed. The hydrodynamic diameters of the formulation were in the range of 140 to 170 nm with a spherical shape and negative zeta potential between −35.3 and −22.8 mV. The EE of formulations were between 70 and 76.4% with slower release measured compared to colistin. Molecular interactions were determined from FTIR and NMR spectra. Molecular docking simulation showed that multiple hydrogen bonds were present between the hydrophilic ring of colistin and SDCS. The colistin:SDCS formulations improved the thermal sensitivity of the mice compared to the control group. The BUN and Cr results showed no significant kidney dysfunction; however, the oxidative stress biomarkers decreased in the colistin with a lesser decrease in colistin-SDCS treated mice. Several histological alterations were observed in the kidney, liver, spleen, and sciatic nerve tissues following colistin treatment, whereas less evidence of toxicity with colistin-SDCS. The overall results indicated that micelle formulations with SDCS showed safer for kidney and nerve cells while maintaining the antibacterial activity of colistin. This study revealed the potential for colistin development with SDCS for safer clinical use against MDR-GNB.
Description: Doctor of Philosophy (Pharmaceutical Sciences), 20232023-01-01T00:00:00ZBiological activities of Gel Containing Kaempferia marginata ExtractThawiwan Muthachanhttp://kb.psu.ac.th:80/psukb/handle/2016/190982023-11-22T02:20:50Z2018-01-01T00:00:00ZTitle: Biological activities of Gel Containing Kaempferia marginata Extract
Authors: Thawiwan Muthachan
Abstract: Kaempferia marginata Carey has been traditionally used in the treatment of inflammation. The ethanol extract exhibited the potent anti-inflammatory properties, therefore this study aimed to investigate biological activities on anti-inflammatory, wound healing and anti-oxidant activities. The extract was developed as gel formulations with the physical, chemical and biological stabilities. The anti-inflammatory activity was tested on anti-nitric oxide (NO) production, the wound healing activities were tested on cell proliferation and migration whereas, anti-oxidant activities were tested on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and hydrogen peroxide (H2O2)-induced oxidative stress. The anti-inflammatory activity of gel containing K. marginata of 10% w/w showed the highest activity with an IC50 value of 12.50 (before) and 12.83 μg/ml (after) (Diclofenac gel, IC50 value = 64.90 μg/ml). The wound healing activities of 5% w/w gel showed the highest % cell viability at 134.05 (before) and 134.65% (after) (Aloe vera gel, % cell viability = 92.64%) and the highest % cell migration was at 85.22 (before) and 85.71% (after) (Aloe vera gel, % cell migration = 72.64%). Moreover, the anti-oxidant activity on H2O2-induced oxidative stress activity assay of 10% w/w gel showed the highest % cell viability at 88.66 (before) and 87.94% (after) (vitamin C and vitamin E, % cell viability = 68.45 and 78.12%). However, the study on DPPH radical scavenging assay of samples were inactive. K. marginata gels showed good chemical stabilities which were evaluated by HPLC analysis. The samples were indicated that % desmethoxyyangonin both before and after accelerating conditions of 2.5% w/w were 75.1 and 78.7% and 5.0% w/w were 77.1 and 81.3%
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whereas at 10.0% w/w (77.7% and 84.8%) was slightly different. K. marginata gels also exhibited the good physical stabilities. The evaluations (color, odor and texture) both before and after accelerating conditions of samples were not different. The pH values of 2.5% w/w were 5.63 and 5.72, 5.0% w/w were 5.41 and 5.91 and 10.0% w/w were 6.42 and 6.78. The viscosity (103cP) values of 2.5% w/w were 7.05 and 7.51 ×103cP, 5.0% w/w were 6.03 and 6.43 ×103cP and 10.0% w/w were 6.07 and 7.06 ×103cP which also were not different.
Gel containing K. marginata extracts have good anti-inflammatory, wound healing and anti-oxidant properties in vitro and showed physical, chemical and biological stabilities after accelerating conditions. The biological properties from this plant could support its traditional uses and obtain a new pharmaceutical product that has physical, chemical and biological stabilities.
Description: Master of Pharmacy (Pharmaceutical Sciences), 20182018-01-01T00:00:00ZAn Antibacterial patch made from mangosteen peel extract / Turawat PhadungkarnTurawat Phadungkarnhttp://kb.psu.ac.th:80/psukb/handle/2016/134942021-03-16T08:03:45Z2011-01-01T00:00:00ZTitle: An Antibacterial patch made from mangosteen peel extract / Turawat Phadungkarn
Authors: Turawat Phadungkarn
Description: Thesis (M.Pharm., Pharmaceutical Sciences)--Prince of Songkla University, 20112011-01-01T00:00:00ZEffects of the missense mutations and the anticancer drug cisplatin on ubiquitination of BRCA1 RING finger domainApichart Atipairinhttp://kb.psu.ac.th:80/psukb/handle/2016/134792021-03-16T06:38:19Z2010-01-01T00:00:00ZTitle: Effects of the missense mutations and the anticancer drug cisplatin on ubiquitination of BRCA1 RING finger domain
Authors: Apichart Atipairin
Description: Thesis (Ph.D., Pharmaceutical Science)--Prince of Songkla University, 20102010-01-01T00:00:00Z